Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice.
Identifieur interne : 003683 ( Main/Exploration ); précédent : 003682; suivant : 003684Immune responses against severe acute respiratory syndrome coronavirus induced by virus-like particles in mice.
Auteurs : Xinya Lu [République populaire de Chine] ; Yao Chen ; Bingke Bai ; Hui Hu ; Ling Tao ; Jihong Yang ; Jianjun Chen ; Ze Chen ; Zhihong Hu ; Hanzhong WangSource :
- Immunology [ 1365-2567 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Femelle, Immunisation (), Immunité cellulaire, Interféron gamma (biosynthèse), Interleukine-4 (biosynthèse), Microscopie électronique, Modèles animaux de maladie humaine, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux, Interféron gamma, Interleukine-4.
- immunologie : Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- Animaux, Femelle, Immunisation, Immunité cellulaire, Microscopie électronique, Modèles animaux de maladie humaine, Souris, Souris de lignée BALB C, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), Disease Models, Animal, Enzyme-Linked Immunosorbent Assay (methods), Female, Immunity, Cellular, Immunization (methods), Interferon-gamma (biosynthesis), Interleukin-4 (biosynthesis), Mice, Mice, Inbred BALB C, Microscopy, Electron, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Viral, Interferon-gamma, Interleukin-4.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, Viral Vaccines.
- methods : Enzyme-Linked Immunosorbent Assay, Immunization.
- Animals, Disease Models, Animal, Female, Immunity, Cellular, Mice, Mice, Inbred BALB C, Microscopy, Electron.
Abstract
Virus-like particles (VLPs) represent a promising vaccine against severe acute respiratory syndrome coronavirus (SARS CoV). In this study, recombinant baculovirus vAcS and vAcME were constructed to express the S protein and the M and E proteins of SARS CoV, respectively. Electron microscope analysis demonstrated the formation of VLPs in vAcME and vAcS coinfected insect cells. Mice immunized four times with VLPs developed high antibody titres against SARS CoV. In addition, VLPs elicited cell-mediated immunity as demonstrated by enhanced interferon-gamma and interleukin-4 production. VLPs also conferred protective immunity against the infection of Spike protein pseudotyped murine leukaemia virus. Our findings demonstrate that SARS CoV VLPs are immunogenic and can elicit strong SARS CoV-specific humoral and cellular immune responses in mice. This is the first study describing the immunogenicity of SARS CoV VLPs, providing valuable data for developing a protective vaccine against SARS CoV infection.
DOI: 10.1111/j.1365-2567.2007.02676.x
PubMed: 17680799
Affiliations:
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first="Ling" last="Tao">Ling Tao</name></author><author><name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name></author><author><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name></author><author><name sortKey="Hu, Zhihong" sort="Hu, Zhihong" uniqKey="Hu Z" first="Zhihong" last="Hu">Zhihong Hu</name></author><author><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name></author></analytic><series><title level="j">Immunology</title><idno type="eISSN">1365-2567</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Disease Models, Animal</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Female</term><term>Immunity, Cellular</term><term>Immunization (methods)</term><term>Interferon-gamma (biosynthesis)</term><term>Interleukin-4 (biosynthesis)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Microscopy, Electron</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Femelle</term><term>Immunisation ()</term><term>Immunité cellulaire</term><term>Interféron gamma (biosynthèse)</term><term>Interleukine-4 (biosynthèse)</term><term>Microscopie électronique</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Test ELISA ()</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Interferon-gamma</term><term>Interleukin-4</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Interféron gamma</term><term>Interleukine-4</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Female</term><term>Immunity, Cellular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Microscopy, Electron</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Immunisation</term><term>Immunité cellulaire</term><term>Microscopie électronique</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Virus-like particles (VLPs) represent a promising vaccine against severe acute respiratory syndrome coronavirus (SARS CoV). In this study, recombinant baculovirus vAcS and vAcME were constructed to express the S protein and the M and E proteins of SARS CoV, respectively. Electron microscope analysis demonstrated the formation of VLPs in vAcME and vAcS coinfected insect cells. Mice immunized four times with VLPs developed high antibody titres against SARS CoV. In addition, VLPs elicited cell-mediated immunity as demonstrated by enhanced interferon-gamma and interleukin-4 production. VLPs also conferred protective immunity against the infection of Spike protein pseudotyped murine leukaemia virus. Our findings demonstrate that SARS CoV VLPs are immunogenic and can elicit strong SARS CoV-specific humoral and cellular immune responses in mice. This is the first study describing the immunogenicity of SARS CoV VLPs, providing valuable data for developing a protective vaccine against SARS CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Bai, Bingke" sort="Bai, Bingke" uniqKey="Bai B" first="Bingke" last="Bai">Bingke Bai</name><name sortKey="Chen, Jianjun" sort="Chen, Jianjun" uniqKey="Chen J" first="Jianjun" last="Chen">Jianjun Chen</name><name sortKey="Chen, Yao" sort="Chen, Yao" uniqKey="Chen Y" first="Yao" last="Chen">Yao Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Hu, Hui" sort="Hu, Hui" uniqKey="Hu H" first="Hui" last="Hu">Hui Hu</name><name sortKey="Hu, Zhihong" sort="Hu, Zhihong" uniqKey="Hu Z" first="Zhihong" last="Hu">Zhihong Hu</name><name sortKey="Tao, Ling" sort="Tao, Ling" uniqKey="Tao L" first="Ling" last="Tao">Ling Tao</name><name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name><name sortKey="Yang, Jihong" sort="Yang, Jihong" uniqKey="Yang J" first="Jihong" last="Yang">Jihong Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Xinya" sort="Lu, Xinya" uniqKey="Lu X" first="Xinya" last="Lu">Xinya Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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